PM4NGS RNASeq Tutorial


This tutorial will show how to create and configure a Google Cloud Platform instance for Next Generation Sequencing (NGS) data analysis. PM4NGS is a program manager for NGS data analysis that is designed to generate a standard organizational structure including directory structures for the project, Jupyter notebooks for data management and CWL workflows for the pipeline execution.

Background reading

  1. Vera Alvarez R, Pongor LS, Mariño-Ramírez L and Landsman D. PM4NGS, a project management framework for next-generation sequencing data analysis, GigaScience, Volume 10, Issue 1, January 2021, giaa141,

  2. Vera Alvarez R, Mariño-Ramírez L and Landsman D. Transcriptome annotation in the cloud: complexity, best practices, and cost, GigaScience, Volume 10, Issue 2, February 2021, giaa163,

Help and Support

For feature requests or bug reports, please open an issue on our GitHub Repository.